The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in anIn VitroCaries Model

Author:

Zaura Egija1,Buijs Mark J.1,Hoogenkamp Michel A.1,Ciric Lena2,Papetti Adele3,Signoretto Caterina4,Stauder Monica5,Lingström Peter6,Pratten Jonathan2,Spratt David A.2,Wilson Michael2

Affiliation:

1. Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands

2. Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK

3. Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy

4. Microbiology Section, Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134 Verona, Italy

5. DIPTERIS, University of Genoa, Corso Europa 26, 16132 Genoa, Italy

6. Department of Cariology, Institute of Odontology at Sahlgrenska Academy, University of Gothenburg, P.O. Box 450, 405 30 Göteborg, Sweden

Abstract

The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) inin vitrocaries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a substratum. The test compounds were low molecular weight fraction (MLMW) of the shiitake extract and subfractions 4 and 5 (SF4 and SF5) of this fraction. Chlorhexidine (CHX) and water served as a positive and a negative control, respectively. Dentin mineral loss was quantified (TMR), microbial shifts within the microcosms were determined (qPCR), and the acidogenicity of the microcosms was assessed (CIA). From the compounds tested, the SF4 of shiitake showed strong inhibiting effect on dentin demineralization and induced microbial shifts that could be associated with oral health. The acid producing potential was increased, suggesting uncoupling of the glycolysis of the microbiota by the exposure to SF4. In conclusion, the results suggest that SF4 of shiitake has an anticariogenic potential.

Funder

European Union’s Sixth Framework Programme

Publisher

Hindawi Limited

Subject

Health, Toxicology and Mutagenesis,Genetics,Molecular Biology,Molecular Medicine,General Medicine,Biotechnology

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