Glucotoxicity Induced Oxidative Stress and InflammationIn VivoandIn VitroinPsammomys obesus: Involvement of Aqueous Extract ofBrassica rapa rapifera

Abstract

Context.Brassica rapais considered as natural source of antioxidants and is used to treat diabetes.Objective. Our study carried the impact of glucotoxicity inducedin vivoandin vitroin vascular smooth muscle cells (VSMCs) inPsammomysand the therapeutic effect ofBrassica rapa(AEBr).Materials and Methods. We administered a hyperglucidic diet (30% sucrose) for 9 months and a treatment for 20 days with AEBr at 100 mg/kg. VSMCs were submitted to D-Glucose (0.6%) for 48 hours and treated with AEBr (2100 μg/mL) for 24 hours. We measured, in blood metabolic parameters, the redox statues and inflammatory markers in adipose tissue. Histological study was effectuated in liver. In VSMCs, we measured markers of glucotoxicity (IRS1p Serine, AKT) inflammation (NO, MCP1, TNFα, and NF-κB) and oxidative stress (oxidants and antioxydants markers). Cell viability and apoptosis were estimated by the morphological study.Results. AEBr corrects the metabolic parameters and inflammatory and oxidative markers in blood and homogenate tissue and reduces lipid droplets in liver. It induces, in VSMCs, a significant decrease of IRS1p serine, cyt c, NO, MCP1, TNFα, NF-κB, protein, and lipid oxidation and increases cell viability, AKT, ERK1/2, catalase, and SOD activity.Conclusion.Brassicaenhanced the antidiabetic, anti-inflammatory, and antioxidant defense leading to the protection of cardiovascular diseases.