Construction and Identification of Eukaryotic Expression Vector pEGFP‐N1‐MIC‐1 for Mouse MIC‐1 Gene and Its Effect on Gastric Cancer Cells

Abstract

This study aimed to construct an eukaryotic expression vector, pEGFP‐N1‐MIC‐1, for overexpressing the mouse macrophage inhibitory cytokine‐1 (MIC‐1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC‐1 gene expression and assess its impact on macrophage phenotype conversion. Enzyme digestion and DNA sequencing confirmed the successful construction of the pEGFP‐N1‐MIC‐1 vector. The transfected MFC cells exhibited a significant increase in MIC‐1 protein expression levels. Furthermore, transfection with pEGFP‐N1‐MIC‐1 increased the migration and colony formation capabilities of MFC cells. These results may contribute to future research and the development of therapeutic interventions targeting MIC‐1 in macrophages, particularly in the context of gastric cancer.