Endogenous Production of n-3 Polyunsaturated Fatty Acids Promotes Fracture Healing in Mice


Chen Yuhui1,Cao He1,Sun Dawei2,Lin Changxin1,Wang Liang1,Huang Minjun1,Jiang Huaji1,Zhang Zhongmin1,Jin Dadi1ORCID,Zhang Baiyu3ORCID,Bai Xiaochun1ORCID


1. Department of Orthopedic, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510665, China

2. Department of Orthopedics & Microsurgery, Guangdong Second Provincial General Hospital, Guangzhou 510317, China

3. Department of Rehabilitation Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China


Bone fracture is a global healthcare issue for high rates of delayed healing and nonunions. Although n-3 polyunsaturated fatty acid (PUFA) is considered as a beneficial factor for bone metabolism, only few studies till date focused on the effects of n-3 PUFAs on fracture healing. In this study, we investigated the effect of endogenous n-3 PUFAs on fracture healing by measuring femur fracture repair in bothfat-1transgenic mice and WT mice. Proximal femoral fracture model was established infat-1transgenic mice and WT mice, respectively, and then the fracture was analyzed by using X-ray, micro-computed tomography (micro-CT), and histological assessment at 7, 14, 21, 28, and 35 days after fixation. The results showed that compared with WT mice,fat-1mice exhibited acceleration in fracture healing through radiographic and histological analysis (18–21 days versus 21–28 days postfracture). Meanwhile, X-ray and micro-CT analysis that showed better remodeling callus formation were in thefat-1group compared to WT group. Furthermore, histological analysis revealed that endogenous n-3 PUFAs promoted local endochondral ossification and accelerated the remodeling of calcified calluses after fracture. In conclusion, the present study indicated that endogenously produced n-3 PUFAs promote fracture healing process and accelerate bone remodeling in mice, and supplementation of n-3 PUFAs was positively associated with fracture healing.


The State Key Development Program for Basic Research of China


Hindawi Limited


Health Informatics,Biomedical Engineering,Surgery,Biotechnology

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