Peptide ARHGEF9 Inhibits Glioma Progression via PI3K/AKT/mTOR Pathway

Author:

Huang Jie1,Fu Xiaoling2,Xue Qiang1ORCID,Ma Peng1,Yin Yating1,Jiang Minjie3,Lu Yunpeng3,Ying Qi1,Jiang Jun4,He Hua1ORCID,Wu Da3ORCID

Affiliation:

1. Department of Neurosurgery, Third Affiliated Hospital, Navy Medical University, Shanghai, 200432, China

2. Department of Medical Psychology, The Fourth Medical Center of PLA General Hospital, 51 Fu Cheng Road, Beijing, 100048, China

3. Department of Neurosurgery, Yixing People’s Hospital, Yixing, Jiangsu Province, 214200, China

4. Endoscopy Center, Minhang Hospital, Fudan University, Shanghai, China

Abstract

Background. The most prevalent malignant tumor in a human brain nervous system is called glioma. Peptide is a compound formed by the peptide bond of α-amino acids, and the development of polypeptide drugs has been widely used in many fields. We plan to investigate the underlying peptides with clinical value in glioma. Method. Based on public databases, we targeted the common genes between glioma differentially expressed genes (DEGs) and peptide genes related to glioma prognosis. Then, these common genes were analyzed by LASSO-Cox analysis, prognostic risk model, and nomogram to identify key prognostic peptide genes and the target gene in this study. Next, the mechanism of target gene in glioma was explored by bioinformatics analysis and functional experiments. Results. We obtained a total of 26 overlapping genes for the following study. After that, 6 independent prognostic factors (REPIN1, PSD3, RDX, CDK4, FANCI, and ARHGEF9) were obtained and applied to construct the prognostic nomogram, and ARHGEF9 was the target gene in the study. Next, peptide ARHGEF9 was found to inhibit glioma cell development. Through Spearman’s correlation analysis, ARHGEF9 had a close relation with PI3K/AKT/mTOR pathway. In functional experiments, peptide ARHGEF9 could suppress the protein expressions of p-PIK3K, p-AKT and p-mTOR, while IGF-1 could reverse this effect. Conclusion. This study identifies 6 new prognostic biomarkers for glioma patients. Among them, peptide ARHGEF9 gene is an inhibitory gene functioning by targeting PI3K/AKT/mTOR pathway.

Publisher

Hindawi Limited

Subject

Biochemistry (medical),Clinical Biochemistry,Genetics,Molecular Biology,General Medicine

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