Identification of Common Genes Refers to Colorectal Carcinogenesis with Paired Cancer and Noncancer Samples-Reference-Cited by-同舟云学术

Identification of Common Genes Refers to Colorectal Carcinogenesis with Paired Cancer and Noncancer Samples

Published:2018 Issue: Volume:2018 Page:1-7
ISSN:0278-0240
Container-title:Disease Markers
language:en
Short-container-title:Disease Markers

Author:

Zhang Lihua¹²^ORCID,Yang Yonglong³^ORCID,Cheng Lin⁴,Cheng Yu¹²^ORCID,Zhou Hong-Hao¹²^ORCID,Tan Zhi-Rong¹²^ORCID

Affiliation:

1. Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China

2. Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China

3. Haikou People’s Hospital and Affiliated Haikou Hospital of Xiangya Medical School, Central South University, Haikou, Hainan 570311, China

4. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 South Xianlie Road, Guangzhou, Guangdong, China

Abstract

Colorectal cancer is a malignant tumor which harmed human beings’ health. The aim of this study was to explore common biomarkers associated with colorectal carcinogenesis in paired cancer and noncancer samples. At first, fifty-nine pairs of colorectal cancer and noncancer samples from three gene expression datasets were collected and analyzed. Then, 181 upregulation and 282 downregulation common differential expression genes (DEGs) were found. Next, functional annotation was performed in the DAVID database with the DEGs. Finally, real-time polymerase chain reaction (PCR) assay was conducted to verify the analyses in sixteen colorectal cancer and individual-matched adjacent mucosa samples. Real-time PCR showed that MCM2, RNASEH2A, and TOP2A were upregulated in colorectal cancer compared with adjacent mucosa samples (MCM2, P<0.001; RNASEH2A, P<0.001; TOP2A, P=0.001). These suggested that 463 DEGs might contribute to colorectal carcinogenesis.

Funder

Haikou Key Scientific and Technological Project

Publisher

Hindawi Limited

Subject

Biochemistry, medical,Clinical Biochemistry,Genetics,Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/dm/2018/3452739.pdf

Reference33 articles.

1. Global cancer statistics, 2012

2. Increased risk of adenomas in individuals with a family history of colorectal cancer: results of a meta-analysis

3. Incidence and Mortality of Colorectal Cancer in Individuals With a Family History of Colorectal Cancer