Quantitative Analysis of Intestinal Flora of Uygur and Han Ethnic Chinese Patients with Ulcerative Colitis

Abstract

Aim. To study the correlation between intestinal flora and ulcerative colitis by analyzing the abundance ofBacteroides, Fusobacterium, Clostridium, Bifidobacteriumspp., andFaecalibacterium prausnitziiin the intestinal of ulcerative colitis (UC) patients and healthy controls with Uygur and Han ethnic.Methods. Bacterial genomic DNA was extracted from fecal samples and analyzed with real-time fluorescence quantitative polymerase chain reaction (PCR) to identify the abundance ofBacteroides, Fusobacterium, Clostridium, Bifidobacteriumspp., andFaecalibacterium prausnitzii.Results. The samples from UC patients, Uygur and Han ethnic combined, had higher abundance ofBacteroides(P=0.026) but lowerClostridium(P=0.004),Bifidobacteriumspp. (P=0.009), andFaecalibacterium prausnitzii(P=0.008) than those from healthy controls. Among UC patients,Bacteroidespopulation was raised in acute UC patients (P≤0.05), while the abundance ofClostridium,Bifidobacteriumspp.,Fusobacterium, andFaecalibacterium prausnitziidecreased (P≤0.05) compared with the remission. In both UC patients group and control group, no difference was observed in the abundance of these 5 bacteria between the Han and the Uygur group.Conclusions. Variations in the abundance of these five bacterial strains in intestines may be associated with the occurrence of UC in Uygur and Han populations; however, these variations were not associated with ethnic difference.