Affiliation:
1. Department of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, Turkey
2. Department of Biological Education, Ahmet Keleşoğlu Education Faculty, Necmettin Erbakan University, 42090 Konya, Turkey
3. Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius
Abstract
We aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium,Onobrychis hypargyrea, andVicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (α-amylase andα-glucosidase), neurodegenerative disorders (tyrosinase, acetylcholinesterase, and butyrylcholinesterase), and hyperpigmentation (tyrosinase). The antioxidant potential, phenolic and flavonoid content of ethyl acetate, and methanolic and aqueous extracts were investigated usingin vitroassays. The total antioxidant capacity (TAC),β-carotene/linoleic acid bleaching activity, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH•), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions were used to evaluate the antioxidant capabilities of the extracts. The half-maximal inhibitory concentrations (IC50) of the extracts on cholinesterase, tyrosinase, andα-amylase were significantly higher than the references, galantamine, kojic acid, and acarbose, respectively. The half-maximal effective concentrations (EC50) of the extracts on TAC, CUPRAC, and FRAP were significantly higher than trolox. The phenol and flavonoid contents of the plant extracts were in the range20.90±0.190–83.25±0.914 mg gallic acid equivalent/g extract and1.45±0.200–39.71±0.092 mg rutin equivalent/g extract, respectively. The plants were found to possess moderate antioxidant capacities and interesting inhibitory action against key enzymes.
Funder
Türkiye Bilimsel ve Teknolojik Arastirma Kurumu
Subject
Pharmacology (medical),General Pharmacology, Toxicology and Pharmaceutics,Molecular Medicine
Cited by
40 articles.
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