Antioxidant Status of Vigna mungo L. in Response to Sulfur Nutrition

Abstract

Black gram (Vigna mungo L. var. DPU-88-31), an edible legume, was grown at 1, 2, 4, 6, and 8 meq S L−1 to study the effect of deficient and excess level of sulfur on oxidative metabolism. Plants supplied by 4 meq S L−1 showed optimum yield. Sulfur deficient plants (1 and 2 meq S L−1) showed reduction in growth and chlorosis of young leaves. Tissue sulfur and cysteine concentration was increased with increasing sulfur supply. The thresholds for critical concentration of sulfur deficiency and toxicity were 0.315% and 0.434% dry weight. Biomass and photoassimilatory pigments were decreased and carbohydrates (sugar and starch) were accumulated in leaves of sulfur deficient and excess plants. Accumulation of hydrogen peroxide and thiobarbituric acid reactive substances in sulfur deficient and excess plants caused oxidative damage in plants which was also evident by the increase in the activity of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione reductase, and concentration of ascorbate and nonprotein thiols.