Characterization of Outer Membrane Vesicles fromBrucella melitensisand Protection Induced in Mice

Author:

Avila-Calderón Eric Daniel1,Lopez-Merino Ahidé1,Jain Neeta2,Peralta Humberto3,López-Villegas Edgar Oliver1,Sriranganathan Nammalwar2,Boyle Stephen M.2,Witonsky Sharon2,Contreras-Rodríguez Araceli1

Affiliation:

1. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Colonia Santo Tomás, 11340, Mexico, DF, Mexico

2. Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060, USA

3. Programa de Genómica Funcional de Procariotes, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Avenue Universidad s/n, P.O. Box 565-A, 62210 Cuernavaca, MOR, Mexico

Abstract

The outer membrane vesicles (OMVs) from smoothB. melitensis16 M and a derived rough mutant, VTRM1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. Proteomic analysis showed 29 proteins present in OMVs fromB. melitensis16 M; some of them are well-knownBrucellaimmunogens such as SOD, GroES, Omp31, Omp25, Omp19, bp26, and Omp16. OMVs from a rough VTRM1 induced significantly higher expression of IL-12, TNFα, and IFNγ genes in bone marrow dendritic cells than OMVs from smooth strain 16 M. Relative to saline control group, mice immunized intramuscularly with rough and smooth OMVs were protected from challenge with virulent strainB. melitensis16 M just as well as the group immunized with live strainB. melitensisRev1 (P<0.005). Additionally, the levels of serum IgG2a increased in mice vaccinated with OMVs from rough strain VTRM1 consistent with the induction of cell-mediated immunity.

Funder

Consejo Nacional de Ciencia y Tecnología

Publisher

Hindawi Limited

Subject

General Medicine,Immunology,Immunology and Allergy

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