Cloning and Embryo Splitting in Mammalians: Brief History, Methods, and Achievements

Author:

Rahbaran Mohaddeseh1,Razeghian Ehsan2,Maashi Marwah Suliman3,Jalil Abduladheem Turki4ORCID,Widjaja Gunawan5,Thangavelu Lakshmi6,Kuznetsova Mariya Yurievna7,Nasirmoghadas Pourya8,Heidari Farid1ORCID,Marofi Faroogh9,Jarahian Mostafa10ORCID

Affiliation:

1. Animal Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

2. Human Genetics Division, Medical Biotechnology Department, National Institute of Genetics Engineering and Biotechnology (NIGEB), Tehran, Iran

3. Stem Cells and Regenerative Medicine Unit at King Fahad Medical Research Centre, Jeddah, Saudi Arabia

4. Faculty of Biology and Ecology, Yanka Kupala State University of Grodno, Grodno, Belarus

5. Universitas Krisnadwipayana, Jakarta, Indonesia

6. Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India

7. Sechenov First Moscow State Medical University, Moscow, Russia

8. Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

9. Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

10. German Cancer Research Center, Toxicology and Chemotherapy Unit (G401), 69120 Heidelberg, Germany

Abstract

Embryo splitting is one of the newest developed methods in reproductive biotechnology. In this method, after splitting embryos in 2-, 4-, and even 8-cell stages, every single blastomere can be developed separately, but the embryos are genetically identical. Embryo splitting, as an approach in reproductive cloning, is extensively employed in reproductive medicine studies, such as investigating human diseases, treating sterility, embryo donation, and gene therapy. In the present study, cloning in mammalians and cloning approaches are briefly reviewed. In addition, embryo splitting and the methods commonly used in embryo splitting and recent achievements in this field, as well as the applications of embryo splitting into livestock species, primate animals, and humans, are outlined. Finally, a perspective of embryo splitting is provided as the conclusion.

Publisher

Hindawi Limited

Subject

Cell Biology,Molecular Biology

Reference51 articles.

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2. Potential of human twin embryos generated by embryo splitting in assisted reproduction and research;L. Noli;Human Reproduction Update,2016

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