Affiliation:
1. Qingdao Fuwai Cardiovascular Hospital, Qingdao, Shandong 266034, China
Abstract
Objective. To determine the level of expression and clinical importance of serum miR-204 and miR-451 in patients with pulmonary arterial hypertension caused by congenital heart disease (CHD-PAH). Methods. From July 2019 to January 2021, 114 infants with congenital heart disease (CHD) were hospitalized at Qingdao’s Fuwai Cardiovascular Hospital. They were grouped into categories: CHD (53 cases) and CHD-PAH (61 cases) based on whether they had pulmonary hypertension (PAH). In addition, 60 healthy children were selected as the control group. All children underwent routine biochemical examination, echocardiography, and pulmonary arterial pressure examination. By using an enzyme-linked immunosorbent assay (ELISA), the levels of brain natriuretic peptide (BNP) and asymmetric dimethylarginine (ADMA) in the blood were measured. Additionally, reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression levels of miR-204 and miR-451 in peripheral blood. The correlation between miR-204, miR-451, BNP, ADMA, and mPAP was investigated using Pearson correlation analysis. Results. Consequently, the TC, BNP, and ADMA serum levels were considerably higher in the CHD and CHD-PAH groups than in the control group (
), whereas BNP and ADMA serum levels were significantly higher in the CHD-PAH group than in the CHD group (
). According to RT-PCR data, the expression levels of miR-204 and miR-451 in the peripheral blood of children in the CHD and CHD-PAH groups were significantly lower (
) when compared to the control group. The expression levels of miR-204 and miR-451 in the peripheral blood of children in the CHD-PAH group were substantially lower (
) than in the CHD group. Significantly, the findings of the ROC curve revealed that the area under the curve (AUC) of CHD-PAH diagnosed by miR-204 and miR-451 alone was 0.737 and 0.725, respectively, and the AUC of joint diagnosis was 0.840, which was greater than that of single diagnosis (
). Patients with CHD-PAH had lower levels of miR-204 and miR-451 in their blood, and this was found to be associated with BNP, ADMA, and mPAP, analyzed by Pearson correlation. Conclusions. Children with CHD-PAH can be diagnosed based on aberrant expressions of miR-204 and miR-451 in their peripheral blood serum. Moreover, CHD-PAH can be diagnosed if the combination of miR-204 and miR-451 is detected as a biomarker, which has a higher diagnostic value.
Subject
Applied Mathematics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Modeling and Simulation,General Medicine
Reference32 articles.
1. The effect of family cooperation model on the quality of life of children with congenital heart disease undergoing interventional therapy;C. Y. Huang;Modern Journal of Integrated Traditional Chinese and Western Medicine,2021
2. Meta-analysis of the incidence of perinatal congenital heart disease in my country;W. Q. Xu;International Journal of Reproductive Health/Family Planning,2020
3. Cause of death in adult patients with congenital heart disease: experiences of a tertiary centre;D. Kecskemeti;European Heart Journal,2021
4. The changing landscape of pulmonary arterial hypertension in the adult with congenital heart disease;A. C. van Dissel;Journal of Clinical Medicine,2017
5. Expression and significance of tumor necrosis factor-like weak inducer of apoptosis in children with congenital heart disease complicated with pulmonary hypertension;T. Cai;Journal of Cardiopulmonary Vascular Disease,2020
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献