Effects of Nogo-A Silencing on TNF-αand IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells

Author:

Zhong Jianbin1,Fan Shengnuo2,Yan Zhenwen2,Xiao Songhua2,Wan Limei1,Chen Chibang1,Zhong Simin1,Liu Lu1,Liu Jun2ORCID

Affiliation:

1. Neurology Department, People’s Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, China

2. Neurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China

Abstract

Parkinson’s disease (PD) is a common degenerative disease that lacks efficient treatment. Myelin-associated neurite outgrowth inhibitor A (Nogo-A) is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD. The study aimed to establish the shRNA expression plasmids of Nogo-A gene and explore the regulatory effects of Nogo-A silencing on the expression of inflammation factor tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) as well as tyrosine hydroxylase (TH) in lipopolysaccharide- (LPS-) stimulated rat PC12 cells. The results showed that both mRNA and protein levels of Nogo-A in pGenesil-nogoA-shRNA group were downregulated. The viabilities of PC12 cells decreased with increase of LPS concentrations. LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects. These results suggested that LPS can activate PC12 cells to secrete inflammatory cytokines and lower the TH expression, which can be regulated by Nogo-A gene silencing. Nogo-A silencing might provide new ideas for PD treatment in the future.

Funder

National Natural Science Foundation of China

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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