Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry

Author:

Chen Liyan1,Luo Zhenchao1,Fu Wenguang1,Liao Xinxin1,Cui Zhonglin1,Zhou Jie1ORCID

Affiliation:

1. Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China

Abstract

Background. Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems.Objective. To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS).Methods. Male Sprague-Dawley rats () were randomly divided into 4 groups (/group): sham operation, day 1, day 3, and day 5. Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver. Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed. GC-MS was used to detect urinary metabolomics.Results. Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated () and then decreased and reached close to normal levels at day 5. GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination. Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (). Principle component analysis showed that 7 metabolites distinguished the day 1 and day 3 groups from the sham group.Conclusions. Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury.

Publisher

Hindawi Limited

Subject

Biochemistry, medical,Clinical Biochemistry,Genetics,Molecular Biology,General Medicine

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