Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells

Author:

Zhao Zhongyan1ORCID,Xu Zhiyu2,Liu Tao1,Huang Shixiong1,Huang Huai3,Huang Xiaoyun4ORCID

Affiliation:

1. Department of Neurology, Hainan General Hospital, Haikou 570311, China

2. Department of Critical Care Medicine, Hainan General Hospital, Haikou 570311, China

3. Neurorehabilitation Dept. 2, Guangzhou General Hospital of Guangzhou Military Command of PLA, Guangzhou 510120, China

4. Department of Neurology, The Affiliated Houjie Hospital, Guangdong Medical University, Dongguan 523945, China

Abstract

Migraine is one of the most common neurological disorders which poses significant socioeconomic burden worldwide. Neuroinflammation and oxidative stress both play important roles in the pathogenesis of migraine. Human urinary kallidinogenase (UK) is a tissue kallikrein derived from human urine. Increasing evidence suggests that UK may protect against ischemic stroke, but UK’s treatment potential against migraine remains to be explored. Immortal BV-2 murine microglial cells were treated with UK (125 nM, 250 nM, and 500 nM) and then given lipopolysaccharides (LPS, 1000 ng/mL). Cell viability of BV-2 cells was tested by the CCK-8 assay. Expressions of tumor necrosis factor-α (TNFα), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were examined with the ELISA method and western blot. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were measured to determine oxidative stress. Our results showed that LPS administration increased the levels of proinflammatory cytokines (TNFα, PGE2, IL-6, and IL-1β) and oxidative stress (ROS and MDA) when compared with the control group and decreased significantly upon introduction with UK. Taken together, UK treatment reduced LPS-induced neuroinflammation and oxidative stress in a dose-dependent manner, which might be a potential treatment of migraine.

Funder

Hainan Association for Science and Technology

Publisher

Hindawi Limited

Subject

Anesthesiology and Pain Medicine,Neurology

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