Antimicrobial Activity of Isoniazid in Conjugation with Surface-Modified Magnetic Nanoparticles against Mycobacterium tuberculosis and Nonmycobacterial Microorganisms

Abstract

Isoniazid, the choice antitubercular agent, has only been employed against Mycobacterium tuberculosis. This study evaluated if the enzyme-mimetic activities of magnetic nanoparticles could accelerate the activation process of isoniazid against mycobacterial and, more importantly, non-mycobacterial microorganisms. First, magnetic nanoparticles were synthesized and coated by lipoamino acid; then, isoniazid was conjugated to synthesized nanoparticles. Antibacterial activities of nanoconjugated isoniazid were evaluated against Mycobacterium tuberculosis and four Gram-positive and Gram-negative nonmycobacterial strains through a microdilution broth process. Results showed that the required amount of isoniazid against Mycobacterium tuberculosis would decrease to 44.8% and 16.7% in conjugation with naked and surface-modified magnetic nanoparticles, respectively. Also, 32 μg/mL and 38 μg/mL of isoniazid in conjugation with naked and surface-modified nanoparticles, respectively, could prevent the growth of Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Hence, the vicinity of magnetic nanoparticles with isoniazid could declare promising aspects of isoniazid antibacterial capabilities.