Multislice Spiral Computed Tomography under Hybrid Iterative Reconstruction Algorithm in Staging Diagnosis of Bladder Cancer and Ceramide Glycosylation

Abstract

This study was to discuss the application of multislice spiral computed tomography (CT) in the staging diagnosis of bladder cancer and the effect of ceramide glycosylation. The hybrid iterative reconstruction algorithm was applied. Immunohistochemistry and western blot were used to detect the normal bladder tissues (30 cases) of GCS in group 1 (100 cases) and group 2. The scanned images of all the research objects were obtained, the images with the iterative reconstruction algorithm were reconstructed, and statistical analysis on the CT value under the algorithm was conducted. The results showed that the image quality, blood vessel sharpness, average image score, signal-to-noise ratio, and radiation dose after the spiral CT and iterative reconstruction algorithm all increased, while the noise value decreased. The optical density value of glucosylceramide synthase in group 2 patients increased by 71%, and the optical density value of group 1 increased by 29%. The optical density expression of glucosylceramide synthase in group 1 patients was significantly higher than that in the control group, and there was a statistical difference between the two ( $P<0.05$ ). Among the results of multislice spiral CT for tumor staging, the lesions larger than 5 cm and in the range of 1.1–2 cm in diameter were more sensitive. In 41 patients, there were multiple lesions. A total of 142 cancer lesions were found. The diameter of the tissue ranged from 0.5 to 6.8 cm, with an average diameter of 2.03 ± 0.35 cm. The optical density of glucosylceramide synthase in the group 1 was 5526, and the optical density in group 2 was 2576. The OD expression of GCS in group 1 was greatly higher in contrast to that in group 2, and there was a statistical difference between the two groups ( $P<0.05$ ). The multislice spiral CT examination under this algorithm found that the diagnosis and staging accuracy of lesions with a diameter greater than 5 cm and tumor diameters in the range of 1.1 to 2 cm was higher. The image processed by the hybrid iterative reconstruction algorithm had good effect, high definition, and accuracy.