Affiliation:
1. Department of Botany, Shah Abdul Latif University, Khairpur, 66020 Pakistan
2. Department of Microbiology, Shah Abdul Latif University, Khairpur, 66020 Pakistan
3. Bone marrow transplantation department, Pir Abdul Qadir Shah Jillani Institute of Medical
Sciences, Gambat Khairpur, Pakistan
4. Department of Plant Biology, University of Illinois at Urbana- Champaign, Urbana, IL61801,
USA
Abstract
Flow cytometry is one of the sophisticated tools with its applications in
different biological disciplines. It is potentially efficient in the characterization of
mixed populations of cells present in biological samples, including blood cells,
lymphocytes, microorganisms, sperms, cancer cells, metabolites, antibodies,
DNA/RNA content, proteins, toxins, plant spores, etc. Flow cytometry is widely
applied in the determination of cellular characteristics and cellular components
profiling like cell size, intracellular pH, DNA, RNA, proteins, surface receptors,
membrane potential, calcium, and others. Currently, flow cytometry is pragmatic in
basic as well as applied plant research and plant industrial applications like plant
breeding. Flow cytometry has been considered a reliable, rapid, efficient, and accurate
tool for analysis of ploidy level and nuclear genome size estimation. It is also subjected
to taxonomy to study population/subpopulation dynamics. Gender determination from
pollen grain is also possible due to flow cytometry.
Publisher
BENTHAM SCIENCE PUBLISHERS