Immunomodulatory Activity of Diterpenes over Innate Immunity and Cytokine Production in a Human Alveolar Epithelial Cell Line Infected with Mycobacterium tuberculosis-Reference-Cited by-同舟云学术

Immunomodulatory Activity of Diterpenes over Innate Immunity and Cytokine Production in a Human Alveolar Epithelial Cell Line Infected with Mycobacterium tuberculosis

Published:2023-09 Issue:6 Volume:16 Page:682-689
ISSN:1874-4672
Container-title:Current Molecular Pharmacology
language:en
Short-container-title:CMP

Author:

Castillo-Maldonado Irais¹^ORCID,Pedroza-Escobar David¹^ORCID,Hernández-Herrera Alejandro David¹^ORCID,Luna-Herrera Julieta²^ORCID,del Rocío González-Martínez Marisela³^ORCID,Prieto-Hinojosa Adria I.⁴⁵^ORCID,Turcios-Esquivel Ana Monica⁵^ORCID,Delgadillo-Guzmán Dealmy⁶^ORCID,Ramírez-Moreno Agustina⁷^ORCID,Bustos-Brito Celia⁸^ORCID,Esquivel Baldomero⁸^ORCID,Vega-Menchaca María-del-Carmen⁹^ORCID

Affiliation:

1. Department of Biochemistry, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Mexico

2. Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México

3. Microbiology Department, Faculty of Medicine, Universidad Autonoma de Coahuila, Torreon, Coahuila, Mexico

4. Department of Molecular Immunobiology, Biomedical Research Centre, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Mexico

5. Tecnológico de Monterrey Campus Laguna, Torreon, Mexico

6. Department of Pharmacology, Faculty of Medicine, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Mexico

7. Faculty of Biological Sciences, Universidad Autonoma de Coahuila Unidad Torreon, Torreon, Mexico

8. Institute of Chemistry, National Autonomous University of Mexico, Exterior Circuit, University City, Mexico City, Mexico

9. Faculty of Chemical Sciences, Universidad Juarez del Estado de Durango, Gomez Palacio, Mexico

Abstract

Background: Mexico has the largest number of the genus salvia plant species, whose main chemical compounds of this genus are diterpenes, these chemical compounds have shown important biological activities such as: antimicrobial, anti-inflammatory and immunomodulatory. Objective: This study aimed to evaluate the immunomodulatory activity of three diterpenes: 1) icetexone, 2) anastomosine and 3) 7,20-dihydroanastomosine, isolated from Salvia ballotiflora, over innate immunity and cytokine production in a human alveolar epithelial cell line infected with Mycobacterium tuberculosis. Method: The immunomodulatory activity of diterpenes over innate immunity included reactive oxygen and nitrogen species (ROS and RNS) induction in response to infection; cytokine production included TNF-α and TGF-β induction in response to infection. Results: The diterpenes anastomosine and 7,20-dihydroanastomosine showed a statically significant (p<0.01) increase of RNS after 36 h of infection and treatment of 2.0 µg/mL. Then, the ROS induction in response to infection showed a consistent statically significant (p<0.01) increase after 12 h of diterpenes treatments. The cell cultures showed an anti-inflammatory effect, in the case of TGF-β induction, in response to infection when treated with the diterpenes. On the other hand, there was not any significant effect on TNF-α release. Conclusion: the diterpenes anastomosine and 7,20-dihydroanastomosine increased the production of RNS after 36 h of infection and treatment. Besides, the three diterpenes increased the production of ROS after 12 h. This RNS and ROS modulation can be considered as an in vitro correlation of innate immunity in response to Mycobacterium tuberculosis infection; and an indicator of the damage of epithelial lung tissue. This study also showed an anti-inflammatory immune response by means of TGF-β modulation when compared with control group.

Publisher

Bentham Science Publishers Ltd.

Subject

General Health Professions