Affiliation:
1. Department of Chemistry, College of Science, University of Baghdad, Al-Jadriya Campus, 10071, Baghdad, Iraq
Abstract
Background::
Antiviral drugs are vital since many viruses can produce fatal infections,
as we have recently seen with the COVID-19 pandemic. Antiviral drugs can battle viruses
at multiple stages of their life cycles, including neuraminidase, nucleic acid synthesis, protease,
and virion fusion or entry [1].
Objective::
Antiviral drugs have poor retention in reversed-phase liquid chromatography, which
makes it difficult to analyze a mixture of antiviral medications using high-performance liquid
chromatography. Using zwitterionic hydrophilic interaction liquid chromatography (ZICHILIC),
the paper highlights the simultaneous quantification of three antiviral drugs as active
constituents in pharmaceutical formulations. Moreover, the influence of the length of the spacer
between the two charges in two stationary phases on the retention behavior of antiviral drugs
has been discussed.
Methods::
Two homemade stationary phases (ZIC1-HILIC and ZIC5-HILIC) were utilized for
the qualitative and quantitative analysis of three antiviral drugs, and UV was used as the detector.
Several chromatographic conditions were examined, such as the organic modifier concentration,
buffer concentration, and pH value.
Results::
After optimizing the parameters, the devised method was applied to analyse three antiviral
medications quantitatively. The initial results demonstrated the current procedure for
separating and determining these three antiviral drugs to be sensitive, robust, and effective.
Consequently, the present method has shown excellent repeatability, a broad linear range (0.1-
16.5 μgml-1), and excellent sensitivity (LOD 0.04-0.072 μgml-1). The RSD value of the method
was less than 1.
Conclusion::
A mixed mode of hydrophilic and ion exchange interactions was the predominant
mode of antiviral medications with two ZIC-HILIC stationary phases. The ZIC5-HILIC stationary
phase had a lower detection and limit of quantitation for three antiviral drugs and a prolonged
retention time compared to the ZIC1-HILIC stationary phase with a shorter chain
length.
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics