Toll-like Receptor 4 Gene Polymorphisms in Chinese Population After Allogeneic Hematopoietic Stem Cell Transplantation
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Published:2021-04-30
Issue:2
Volume:16
Page:225-229
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ISSN:1574-8936
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Container-title:Current Bioinformatics
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language:en
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Short-container-title:CBIO
Author:
Zhao Yi1,
Zhang Yujie1,
Zhou Jie1,
Wang Lijuan1,
Shi Jimin1,
Tan Yamin1,
Luo Yi1,
Huang He1,
Cai Zhen1
Affiliation:
1. Department of Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China
Abstract
Objectives:
Graft-versus-host disease (GVHD) is the most common complication after
hematopoietic stem cell transplantation (HSCT) and remains to be a major cause of mortality.
Activation of toll-like receptor 4 (TLR-4) by lipopolysaccharide induces the NF-κB signaling
pathway to release critical proinflammatory cytokines and increases the recipient response to
GVHD. In order to clarify the role of TLR-4 in the occurrence of acute GVHD after HSCT, we
collected 208 samples from HSCT recipients and their human lecucyte antigen identical donors to
test the hypothesis that TLR-4polymorphism in the recipients or donors influence the risk of acute
GVHD in allogeneic HSCT recipients.
Methods:
TLR-4 Asp299Gly and Thr399Ile polymorphisms of each sample were examined by
using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism
methods.
Results:
No homozygous or heterozygous variant alleles of the Asp299Gly or Thr339Ile
polymorphism were detected in any samples in our study. Our results demonstrate that TLR-4
Asp299Gly and Thr399Ile polymorphisms might be very rare in the Chinese population (Eastern
China and Taiwan region).
Conclusion:
The results of this study cannot confirm the role of TLR-4 mutations in the
pathogenesis of GVHD in humans, yet we reach a definite conclusion by a TLR-4 knockout
murine GVHD model in our ongoing project.
Funder
Technology & Ministry of Health
National Natural Science Foundation of China
Publisher
Bentham Science Publishers Ltd.
Subject
Computational Mathematics,Genetics,Molecular Biology,Biochemistry