Humic Acid Derived from Agricultural Biomass Mitigates Alveolar Bone Loss and Modulates Systemic Inflammatory Cytokines in Rats with Periodontitis-Reference-Cited by-同舟云学术

Humic Acid Derived from Agricultural Biomass Mitigates Alveolar Bone Loss and Modulates Systemic Inflammatory Cytokines in Rats with Periodontitis

Published:2024-05-20 Issue: Volume:24 Page:
ISSN:1568-0266
Container-title:Current Topics in Medicinal Chemistry
language:en
Short-container-title:CTMC

Author:

Orlando Patrícia Ribeiro¹,Tavares Hugo Giordano¹,Pereira Ramona Ramalho de Souza¹^ORCID,Silva Gabriela¹,Carvalho Jaqueline do Carmo Lima²^ORCID,Machado Alan Rodrigues Teixeira²^ORCID,Dobbs Leonardo Barros³^ORCID,Dias-Peixoto Marco Fabrício¹^ORCID,Pereira Luciano José⁴^ORCID,Andrade Eric Francelino¹⁴^ORCID

Affiliation:

1. Health Sciences Program, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil

2. Department of Exact Sciences, Universidade Estadual de Minas Gerais, João Monlevade, Minas Gerais, 35930-314, Brazil

3. Institute of Agrarian Sciences, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Unaí, Minas Gerais, Brazil

4. Health Sciences Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil

Abstract

Background: Humic acid (HA) is a bioproduct that can be extracted from different sources and has anti-inflammatory properties that have been little explored in the treatment and prevention of Periodontal Disease (PD). Thus, we aimed to investigate the effects of oral administration of HA on the progression of PD in rats. Methods: Twenty-four male Wistar rats were distributed into three experimental groups (Control/ Sham, PD, and PD + HA). HA was administered by gavage (80 mg/kg/day) for 28 days, and PD was induced 14 days after the beginning of treatment. Bone loss, bone topography, and surface elemental composition were analyzed. Circulating IL1-beta, TNF-alpha, and IL-10 levels were evaluated through Enzyme-Linked Immunosorbent Assay (ELISA). Results: The animals treated with HA showed lower bone loss (p < 0.05). Calcium and phosphorus levels on the alveolar bone surface were lower in the PD group (p < 0.05) compared to the control group, whereas the animals treated with HA exhibited attenuation in this loss (p < 0.05). The animals treated with HA showed reduced TNF-alpha, IL1-beta, IL-10, and the TNF-alpha/IL-10 ratio compared to those with PD (p < 0.05). Conclusion: Treatment with HA attenuated the parameters of alveolar bone loss and modulated systemic inflammatory parameters in rats with ligature-induced PD.

Publisher

Bentham Science Publishers Ltd.