Affiliation:
1. ollege of Basic Medicine, Jinzhou Medical University, JinZhou, Liaoning Province, 121000, China
2. Institute of Materia Medica,
Jinzhou Medical University, JinZhou, Liaoning Province, 121000, China
Abstract
Background:
It is widely recognized that atherosclerosis (AS) is related to vascular inflammation.
Panax notoginseng saponins (PNS) extracted from the roots of Panax notoginseng have been shown to possess
anti-inflammatory activity. It is widely used in the clinical treatment of cardiovascular and cerebrovascular diseases,
but the protective effect of PNS on atherosclerosis is not fully understood. This study was designed to
test the effects of PNS administration in apolipoprotein (apo)-E-deficient (ApoE-/-) mice on the activation of
NF-κB p65, IL-1β, IL-6, TNF-α and Calpain1 proteins.
Methods:
24 ApoE-/- mice fed with high-fat diet for 8 weeks to create the AS model. PNS, dissolved in three
distilled water, was administered orally to two treatment groups at dosages of 60 mg/kg/d/mice and 180
mg/kg/d/mice. After 8 weeks, peripheral blood was collected for assessing the levels of TG, TC, LDL-C and
HDL-C in serum by Biochemical Analyzer. HE staining was used to observe pathomorphological changes in
the aortic root. Oil Red O staining was used to observe the lipid deposition in the aortic root. ELISA kits were
used to assess the levels of IL-1β and TNF-α in serum. The expression levels of NF-κB p65, IL-1β, IL-6,
TNF-α, and Calpain1 proteins in the aortic root were identified by Western blot.
Results:
After PNS administration for 8 weeks, the levels of TG, TC, LDL-C, IL-1β and TNF-α were decreased,
the level of HDL-C was increased in apoE-/- mice. The arrangement of the tissue of aortic root tended
to be normal, the cell morphology was restored, and the lipid depositions were reduced in apoE-/- mice treated
with PNS. Moreover, PNS inhibited the expression levels of NF-κB p65, IL-6, IL-1β, TNF-α and Calpain1 proteins
of aortic root tissues in apoE-/- mice.
Conclusion:
PNS may inhibit the progression of atherosclerotic lesions via their anti-inflammatory biological
property. PNS suppress the NF-κB signaling pathway and inhibits the expression of pro-inflammatory factors
such as NF-κB p65, IL-6, IL-1β, TNF-α and Calpain1 proteins in aortic root tissues of apoE-/- mice.
Funder
Innovative Talents Support Project of Higher Education Institutions in Liaoning Province
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmacology
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