Effects of Huangkui Capsule on the Expression of SPARC in the Kidney Tissue of a Rat Model with Diabetic Nephropathy-Reference-Cited by-同舟云学术

Effects of Huangkui Capsule on the Expression of SPARC in the Kidney Tissue of a Rat Model with Diabetic Nephropathy

Published:2019-11-18 Issue:4 Volume:19 Page:211-215
ISSN:1566-5232
Container-title:Current Gene Therapy
language:en
Short-container-title:CGT

Author:

Yang Xiaoyao¹,Luo Meng²,Jiang Qinghua²,Wang Yiwei¹

Affiliation:

1. Department of Science and Education, Heilongjiang Provincial Hospital, Harbin, China

2. School of Life Science and Technology, Harbin Institute of Technology, Harbin, China

Abstract

Objective: The objective of the research is to investigate the effects of Huangkui capsule on the expression of SPARC in the kidney tissues of diabetic nephropathy. Methods: SD rats were divided into three groups: normal control group, untreated DN group and HKC-treated DN group. The therapeutic effects and underlying molecular mechanism of HKC on DN rats induced by streptozotocin were evaluated by the levels of serum creatinine, blood urea nitrogen, 24-hour urinary protein and the expression of SPARC. Pathological changes in kidney tissues were observed through hematoxylin-eosin (HE) staining. Moreover, western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the variation of SPARC. Results: This study was performed to investigate the effects of HKC on DN in SD rats model and its molecular mechanism. Our results showed that the rats treated with HKC had an improved general state and reduced creatinine, blood urea nitrogen and 24-hour urinary protein levels. The deterioration of renal function was delayed due to treatment with HKC. HE staining was utilized to observe that HKC can improve histopathological findings in the kidney tissues of DN rats, including kidney fibrosis. Results of western blot and qRT-PCR showed that HKC can inhibit the expressions of SPARC in the rat model of DN. Conclusion: The present findings demonstrated that HKC inhibited SPARC level and had significant therapeutic effects on DN.

Funder

Heilongjiang Provincial Health and Family Planning Commission Foundation of China

Harbin Science and Technology Foundation of China

Publisher

Bentham Science Publishers Ltd.

Subject

Genetics (clinical),Drug Discovery,Genetics,Molecular Biology,Molecular Medicine

Reference28 articles.

1. Adeshara K.A.; Diwan A.G.; Tupe R.S.; Diabetes and complications: Cellular signaling pathways, current understanding and targeted therapies. Curr Drug Targets 2016,17(11),1309-1328

2. Hudson B.G.; Tryggvason K.; Sundaramoorthy M.; Neilson E.G.; Alport’s syndrome, Goodpasture’s syndrome, and type IV collagen. N Engl J Med 2003,348(25),2543-2556

3. Cheng L.; Wang P.; Tian R.; LncRNA2Target v2.0: A comprehensive database for target genes of lncRNAs in human and mouse. Nucleic Acids Res 2019,47(D1),D140-D144

4. Cheng L.; Hu Y.; Sun J.; Zhou M.; Jiang Q.; DincRNA: A comprehensive web-based bioinformatics toolkit for exploring disease associations and ncRNA function. Bioinformatics 2018,34(11),1953-1956

5. Lane T.F.; Sage E.H.; The biology of SPARC, a protein that modulates cell-matrix interactions. FASEB J 1994,8(2),163-173

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