Affiliation:
1. Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, China
2. Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming,
Yunnan 650101, China
Abstract
Objective:
The aim of the study was to investigate the therapeutic potential of Berbamine-loaded lipid nanoparticles
(BBM-NPs) in pancreatic cancer.
Methods:
Dopamine polymerization-polylactide-TPGS nanoparticles were synthesized to prepare BBM-NPs, and the
change in particle size of BBM-NPs was measured. Cell Counting Kit-8 (CCK8) assay, plate cloning experiment, and
apoptosis analysis were performed to evaluate the cytotoxicity of BBM-NPs against the pancreatic cancer cells
(PANC-1 and AsPC-1). Migration and invasion abilities of the tumor cells were determined by Transwell and wound
healing assays. The intracellular level of ROS and expression of tumor progression-related proteins were measured
using ROS-kit and western blot assay. Besides, an in vivo study was performed in the Balb/c nude mice to analyze the
function of BBM-NPs in tumor growth.
Results:
The in vitro studies showed that BBM-NPs with stable particle size and sustained drug release effectively
inhibited the viability, proliferation, migration, and invasion of pancreatic cancer cells, while promoting cell apoptosis.
Moreover, the in vivo experiments revealed that compared to Free BBM, BBM-NPs exhibited a stronger inhibitory
effect on the growth of xenograft tumors derived from PANC-1 cells in mice. In addition, increased expressions of
ROS, Bax, Cleaved Caspase-3, and γ-H2AX, as well as decreased expressions of MMP2, MMP9 and Bcl-2 were identified
in both Free BBM and BBM-NPs groups, while BBM-NPs exhibited a stronger effect on protein expression than
Free BBM.
Conclusion:
In summary, BBM-loaded lipid nanoparticles enhanced the therapeutic effects of BBM on pancreatic
cancer, providing a promising strategy for targeted cancer therapy.
Funder
Yunnan Provincial Science and Technology Department Joint Special Project
Major Science and Technology Projects in Yunnan Province
Publisher
Bentham Science Publishers Ltd.
Subject
Cancer Research,Pharmacology,Molecular Medicine
Cited by
4 articles.
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