Affiliation:
1. Department of Dermatology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, China
Abstract
Background:
MIR155HG is a long non-coding RNA (lncRNA) that has been shown to be
dysregulated in a range of tumor types, but the functions of this lncRNA in melanoma remain to be explored.
Objectives:
We explored the functions of lncRNA MIR155HG in melanoma progression.
Methods:
The expression of miR155HG was analyzed in clinical melanoma. Bioinformatics analysis was
performed to assess the potential tumor-related functions of miR155HG. The interaction of miR155HG and SP1
and the inhibition of PSIP1 by miR-485-3p were analyzed by ChIP, luciferase reporter experiments, and the
biological effects in melanoma were explored by colony formation assays, EdU cell proliferation assays,
Transwell analysis, and intracranial melanoma mouse model.
Results:
Herein, we found that MIR155HG was markedly upregulated in melanoma cell lines and tissues. We
further determined that the SP1 transcription factor was responsible for driving MIR155HG upregulation in
melanoma. Elevated MIR155HG levels were linked to decreased overall survival (OS) in melanoma patients, and
we further determined that MIR155HG expression was an independent predictor of melanoma patient prognosis.
When MIR155HG was knocked down in melanoma cells, this impaired their proliferative, migratory, and
invasive activity. By using predictive bioinformatics analyses, we identified miR-485-3p as a microRNA
(miRNA) capable of binding to both MIR155HG and the 3’ UTR of PSIP1.
Conclusion:
Together, these results suggest that MIR155HG is capable of promoting melanoma cell proliferation
via the miR-485-3p/PSIP1 axis. These novel findings provide new insights into the development of melanoma,
potentially highlighting future avenues for therapeutic intervention.
Funder
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities, Xi’an Jiaotong University Campus Project
Publisher
Bentham Science Publishers Ltd.
Subject
Cancer Research,Pharmacology,Molecular Medicine
Cited by
3 articles.
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