Apolipoprotein A1 inhibits adipogenesis progression of human adipose-derived mesenchymal stem cells

Abstract

Background: According to the reports, the most vital characteristic of obesity is aberrant accumulation of triglyceride (TG) in the adipocyte. On the other hand, circulating concentrations of apolipoprotein A1 (apoA1) have been demonstrated to be strongly correlated with the prevalence and the pathological development of obesity. Nevertheless, the underlying mechanisms whereby apoA1 modulates the pathogenesis of obesity is still not fully elucidated. Methods: Adipose-derived mesenchymal stem cells (AMSCs, isolated from the hospitalized patients were intervened with 15 μg/ml recombined human apoA1 protein. The effects of apoA1 in modulating the intracellular levels of TG and the expression contents of adipogenic related cytokines were also analyzed. Furthermore, whether apoA1 modulated the adipogenesis progression was via sortilin was also explored in the current research. Results: During the adipogenesis progression, apoA1 could significantly lower the quantity of intracellular lipid droplets (LDs). Meanwhile, apoA1 could decrease the intracellular levels of TG and down-regulate the expression contents of several vital adipogenic related cytokines, such as CCAAT enhancer binding proteins α/β (C/EBPα/β), fatty acid synthetase (FAS), and fatty acid binding protein 4 (FABP4). Moreover, the inhibitory effect of apoA1 was further verified to be induced through up-regulating the SORT1 gene expression which subsequently increased sortilin protein. Consistent with these findings, silencing the SORT1 gene expression could induce the loss-of-function (LOF) of apoA1 in modulating the adipogenesis progression of AMSCs. Conclusions: In conclusion, apoA1 could suppress the adipogenesis progression of human AMSCs through, at least partly, up-regulating the SORT1 gene expression which subsequently increasing the sortilin protein content. Thereby, the present research sheds light on a novel pathogenic mechanism by which apoA1 regulates adipogenesis progression and proposes that apoA1 embraces the function to treat obesity in clinical practice.