Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia
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Published:2019-07-24
Issue:2
Volume:18
Page:126-141
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ISSN:1871-5230
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Container-title:Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry
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language:en
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Short-container-title:AIAAMC
Author:
Tanod Wendy Alexander1, Yanuhar Uun1, Maftuch 1, Putra Masteria Yunovilsa2, Risjani Yenny1
Affiliation:
1. Faculty of Fisheries and Marine Science, Postgraduate Program, Brawijaya University, Malang, East Java, 65145, Indonesia 2. Research Center for Oceanography, Indonesian Institute of Sciences, Jakarta, 14430, Indonesia
Abstract
Background:
As a marine organism, soft corals can be utilized to be various
bioactive substances, especially terpenoids and steroids. The soft corals family which produces
bioactive generally come from clavulariidae, alcyoniidae, nephtheidae and xeniidae
family.
Objective:
To investigate the bioactivity of Nitric Oxide (NO) inhibitor release from soft
coral crude extracts of Sinularia sp. (SCA), Nephthea sp. (SCB), Sarcophyton sp. (SCC),
Sarcophyton sp. (SCD), Sinularia sp. (SCE) and Sinularia sp. (SCF).
Materials and Methods:
Soft coral is collected from Palu Bay (Central Sulawesi). NO inhibitory
release activity measured according to the Griess reaction. Soft corals sample
macerated with 1:2 (w/v). Then, Soft coral extracts with the best NO Inhibitor activity partitioned
with Dichloromethane, Ethyl acetate, and n-butanol. The bioactive of all crude extracts
were identified by GC-MS to find compounds with anti-inflammatory potential.
Results:
Sarcophyton sp. (SCC) and Sinularia sp. (SCF) are able to inhibit NO concentrations
of 0.22 ± 0.04 and 0.20 ± 0.04 µM at 20 mg/mL, respectively. The chemical constituents
determined and showed the potential as anti-inflammatory in the crude of Sinularia sp.
(SCA) were Octacosane (3.25%). In Nephthea sp., (SCB) were Cyclohexene, 6-ethenyl-6-
methyl-1-(1-methylethyl)-3-(1-methylethylidene)-,(S)- (0.55%); Azulene, 1,2,3,4,5,6,7,8-
octahydro-1,4-dimethyl-7-(1-methylethylidene)-, (1S-cis)- (0.53%); and 1,7,7-Trimethyl-
2-vinylbicyclo[2.2.1]hept-2-ene (4.72%). In Sarcophyton sp, (SCC) were Eicosane
(0.12%); Nonacosane (10.7%); 14(β)-Pregnane (0.87%); Octacosane 6.39%); and Tricosane
(1.53%). In Sarcophyton sp. (SCD) were 14(β)-Pregnane (2.69%); and Octadecane
(27.43%). In crude of Sinularia sp. (SCE) were Oleic Acid (0.63%); 7,10-Hexadecadienoic
acid, methyl ester (0.54%); 14(β)-Pregnane (1.07%); 5,8,11,14-Eicosatetraenoic acid, ethyl
ester, (all-Z)- (4.60%); Octacosane (7.75%); and 1,2-Benzisothiazole, 3-(hexahydro-1Hazepin-
1-yl)-, 1,1-dioxide (1.23%). In the crude of Sinularia sp., (SCF) were Oxirane,
decyl- (1.38%); Nonacosane (0.57%); Cyclohexanol, 5-methyl-2-(1-methylethenyl)-
(0.61%); 14B-Pregnane (0.76%); and Tetratriacontane (1.02%).
Conclusion:
The extract of Sarcophyton sp. (SCC) and Sinularia sp. (SCF) showed the
best NO inhibitory release activity. This study is making soft corals from Central Sulawesi,
Indonesia can become a potential organism in the discovery and development of bioactive
substances anti-inflammatory.
Funder
Ministry of Research, Technology and Higher Education
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology,General Medicine,Immunology,Immunology and Allergy
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