Affiliation:
1. Department of Chemistry and Biochemistry, Institute of Bioscience, UNESP, Botucatu, SP, Brazil
Abstract
Background:
Prostate cancer cells have very high PCA3 messenger RNA levels,
which turns them into one of the new biomarkers for prostate cancer prognosis and diagnosis.
Objective:
Our goal here is to develop a new aptasensor to detect PCA3 release by the
cancer cell.
Methods:
DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methylene
blue (MB) redox probe, and immobilized on gold electrode through self-assembly to
detect label-free cancer cells.
Results:
Our data have evidenced stable and sensitive sensors presenting a wide linear detection
range (0-150ng/mL). In addition, monitoring PCA3 released by different types of
prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore,
it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary depending
on the type of adopted prostate cells.
Conclusion:
PCA3 release was monitored in a group of cells for 2 h; it showed significantly
higher expression in both LNCaP and PC-3 cells. This strategy provides a unique
and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it
emerged as a promising tool for early cost-effective diagnosis
Funder
FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology,Molecular Medicine,Drug Discovery,Biochemistry,Organic Chemistry
Cited by
4 articles.
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