Affiliation:
1. Department of Biotechnology, Prathyusha Engineering College, Thiruvallur, 602025, Tamil Nadu, India
Abstract
Introduction:
Skin cancer is the most common type of cancer caused by the uncontrolled
growth of abnormal cells in the epidermis and the outermost skin layer.
Aim:
This study aimed to study the anti-skin cancer potential of [6]-Gingerol and 21 related structural
analogs using in vitro and in silico studies.
Method:
The ethanolic crude extract of the selected plant was subjected to phytochemical and
GC-MS analysis to confirm the presence of the [6]-gingerol. The anticancer activity of the extract
was evaluated by MTT (3-[4, 5-dimethylthiazol-2-y]-2, 5-diphenyl tetrazolium bromide) assay
using the A431 human skin adenocarcinoma cell line.
Result:
The GC-MS analysis confirmed the presence of [6]-Gingerol compound, and its promising
cytotoxicity IC50 was found at 81.46 ug/ml in the MTT assay. Furthermore, the in silico studies
used [6]-Gingerol and 21 structural analogs collected from the PubChem database to investigate
the anticancer potential and drug-likeliness properties. Skin cancer protein, DDX3X, was selected
as a target that regulates all stages of RNA metabolism. It was docked with 22 compounds,
including [6]-Gingerol and 21 structural analogs. The potent lead molecule was selected based on
the lowest binding energy value.
Conclusion:
Thus, the [6]-Gingerol and its structure analogs could be used as lead molecules
against skin cancer and future drug development process.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Molecular Medicine,General Medicine