Affiliation:
1. Laboratoire Des Sciences Fondamentales, Université Amar Telidji, Laghouat 03000, Algérie
2. Département De Biologie, Université
Amar Telidji, Laghouat 03000, Algérie
3. Laboratoire des Sciences
Appliquées et Didactiques, Ecole Normale Supérieure de Laghouat 03000, Algérie
Abstract
Objective:
The present study aimed to identify new selective inhibitors for acetylcholinesterase,
butyrylcholinesterase, monoacylglycerol lipase, beta-secretase, and Asparagine endopeptidase,
the targets enzymes in Alzheimer’s disease.
Methods:
The inhibitory effect of P. atlantica Desf. methanol extracts against AChE were determined
using Ellman’s method. The molecular docking study is achieved using Autodock Vina. The structures
of the molecules 3-methoxycarpachromene, masticadienonic acid, 7-ethoxycoumarin, 3′,5,7-
trihydroxy-4′-methoxyflavanone and 5,6,7,4′-tetrahydroxyflavonol-3-O-rutinoside and the five enzymes
were obtained from the PubChem database and Protein databank. ADMET parameters were
checked to confirm their pharmacokinetics using swiss-ADME and ADMET-SAR servers.
Results:
P. atlantica Desf. methanol extracts showed a notable inhibitory effect against AChE (IC50 =
0.26 ± 0.004 mg/ml). The molecular docking results of 3-methoxycarpachromene, masticadienonic acid,
7-ethoxycoumarin, 3′,5,7-trihydroxy-4′-methoxyflavanone and 5,6,7,4′-tetrahydroxyflavonol-3-Orutinoside
with the five enzymes show significant affinities of these molecules towards Alzheimer disease
targets, where they could form several interactions, such as hydrogen bonds and hydrophobic interactions
with the studied enzymes. The shortest hydrogen bond is 1.7 A° between masticadienonic
acid and Arg128 of the active site of BACE, while the lowest free energy is -11.2 of the complex
5,6,7,4′-tetrahydroxyflavonol-3-O-rutinoside –HuBchE. To the best of our knowledge, these molecules'
potential anti-Alzheimer disease effect is studied in this paper for the first time.
Conclusion:
The docking studies of this work show that 3-methoxycarpachromene and masticadienonic
acid, 7-ethoxycoumarin, 3′,5,7-Trihydroxy-4′-methoxyflavanone and 5,6,7,4′-tetrahydroxyflavonol-
3-O-rutinoside have good affinities towards the enzymes involved in Alzheimer pathology,
which confirm the ability of these molecules to inhibit the studied enzymes namely: HuAChE, HuBChE,
BACE, MAGL, and AEP. These molecules might become drug candidates to prevent Alzheimer's
disease.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Molecular Medicine,General Medicine