Affiliation:
1. Department of Neurosurgery,
Zhejiang Provincial Hospital of Traditional Chinese Medicine, the First Affiliated Hospital of Zhejiang Chinese Medical
University, Hangzhou, China
2. First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China
Abstract
Objectives:
Melatonin (MT) is a pineal hormone with antineoplastic potential. This study
aims to explore the therapeutic potential and mechanism of MT on glioblastoma (GBM).
Methods:
A human GBM cell line, LN229, was used to evaluate the function of MT. Cell viability,
apoptosis, and migration were detected by CCK-8, flow cytometry, and transwell assays, respectively.
The mRNA and protein expressions of specific genes were measured by qRT-PCR and western
blot, respectively. The regulatory relationship between miR-16-5p and PIM1 was validated by dual
luciferase reporter gene assay. A mouse xenograft model was established to prove the anti-tumor
effect and related mechanisms of MT in vivo.
Results:
MT inhibited the viability and migration and promoted the apoptosis of LN229 cells in a
dose-dependent manner. MiR-16-5p was dose-dependently up-regulated by MT in LN229 cells,
negatively regulating its target PIM1. MiR-16-5p inhibitor eliminated the anti-tumor effect of MT in
LN229 cells, while si-PIM1 reversed the effect of miR-16-5p inhibitor in MT-treated cells. MT
inhibited the tumor growth in vivo and MT-induced PIM1 down-regulation was reversed by miR-
16-5p inhibition in tumor tissues.
Conclusions:
MT inhibits the malignant progression of GBM via regulating miR-16-5p-mediated
PIM1.
Publisher
Bentham Science Publishers Ltd.
Subject
Cellular and Molecular Neuroscience,Developmental Neuroscience,Neurology
Cited by
1 articles.
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