Affiliation:
1. College of Basic Medicine, Guangxi Medical University, Nanning, 530021, China
2. Department of Ultrasound
Diagnosis, 923 Hospital of the People’s Liberation Army, Nanning, 530021, China
Abstract
Introduction:
Diabetic peripheral neuropathy (DN) is the most common complication of
type 2 diabetes mellitus (T2DM).
Objective:
This study aimed to explore the role of fibrinogen (FIB) in T2DM neuropathy and its
preliminary mechanism.
Methods:
Ten male Sprague-Dawley rats were divided into a normal control group (NC group) and a
T2DM neuropathy model group (DN group). The DN group was given a high-energy diet and
streptozotocin, while the NC group was given a normal diet and a citric acid buffer. The expression
levels of related proteins were analysed.
Results:
Electrophysiology: Compared with the NC group, the conduction latency of the
somatosensory-evoked potential and nerve conduction velocity was prolonged in the DN group, while
the motor nerve action potential was decreased. As seen under a light microscope, the peripheral
nerve fibres in the DN group were swollen, and the nerve fibres in the posterior funiculus of the
spinal cord were loose or missing. Moreover, as seen under an electron microscope, the peripheral
nerve demyelination of the DN group was severe, with microvascular blood coagulation, luminal
stenosis, and collapse. Compared with the NC group, in the DN group, the expression of FIB was
positively correlated with the expression of both ionised calcium-binding adaptor molecule-1 and
glial fibrillary acidic protein. Compared with the NC group, in the DN group, the expression of
platelet/endothelial cell adhesion molecule-1 and B-cell lymphoma 2 was negatively correlated.
Conclusion:
The increased concentration of FIB may be the cause of neuropathy, and its mechanism
may be related to its promotion of inflammatory response, blood coagulation, and vascular stenosis.
Funder
National Natural Science Foundation of China
Health Commission of Guangxi Zhuang Autonomous Region
Publisher
Bentham Science Publishers Ltd.
Subject
Biochemistry,General Medicine,Structural Biology
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