Affiliation:
1. Department of Urology, Shantou Central Hospital, Jinping District, Shantou 515031, China
Abstract
Background::
microRNA-628-5p (miR-628-5p) has a significant impact on certain types of cancer.
The precise function of miR-628-5p in the context of bladder urothelial carcinoma (BLCA) remains ambiguous.
Objective::
We aimed to investigate the role of miR-628-5p in BLCA.
Methods::
The samples were collected from The Cancer Genome Atlas (TCGA). Statistics were employed to
evaluate the correlation and predictive significance of miR-628-5p. We analyzed the target genes and regulatory
network of miR-628-5p and the correlation between miR-628-5p and immune infiltration. The expression
of miR-628-5p in BLCA cells was confirmed by quantitative reverse-transcription PCR (qRT-PCR).
Results::
miR-628-5p exhibited differential expression across various types of cancer. There was a significant
association between high expression of miR-628-5p and primary therapy outcome (p < 0.05). High expression
of miR-628-5p was observed to be associated with poorer overall survival (HR: 1.42; 95% CI: 1.06-1.90; p =
0.02), progress free survival (HR: 1.57; 95% CI: 1.17-2.11; p = 0.003), and disease specific survival (HR:
1.83; 95% CI: 1.28-2.62; p = 0.001) in BLCA. miR-628-5p was an independent prognostic factor in BLCA
and may be involved in the development of the disease through various pathways, including focal adhesion,
ECM-receptor interaction, PI3K-Akt signaling pathway, and MAPK signaling pathway, and among others.
miR-628-5p expression was significantly correlated with immune infiltration in BLCA patients. Compared to
normal bladder epithelial cells, BLCA cell lines exhibited a significant upregulation of miR-628-5p.
Conclusion::
It is possible that miR-628-5p could serve as a hopeful therapeutic target and prognostic biomarker
for individuals with BLCA.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmacology