Affiliation:
1. Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
2. Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
Abstract
Background:
Liposomes are mostly known to be prepared from phospholipids and lipids and
have a remarkable capacity to encapsulate both lipophobic and lipophilic molecules. However, there is
little research on developing fatty acid liposomes for chemotherapy.
Objective:
We have successfully prepared mixed fatty acid liposomes from two monounsaturated fatty
acids, namely oleic acid and erucic acid, which stabilised by DOPEPEG2000. The Critical Vesicular
Concentration (CVC) of liposomes was found to be within 0.09 to 0.21 mmol dm-3, with an average
particle size of 400 nm.
Methods:
Encapsulation of various anticancer drugs such as folinic acid, methotrexate, doxorubicin, or
irinotecan resulted in Encapsulation Efficiency (%EE) of up to 90%. Using a 3-(4, 5-dimethylthiazol-2-
yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the median Inhibitory Concentration (IC50) values of
mixed oleic acid-erucic acid encapsulating hydrophilic drugs was remarkably reduced at the end of 24
hours of incubation with the human lung carcinoma cell line A549.
Results:
The results suggest that mixed oleic acid-erucic acid liposomes are a potential new approach to
further develop as an alternative vehicle of various drugs for cancer treatment.
Funder
Prototype Research Grant Scheme
Geran Penyelidikan Fakulti
Fundamental Research Grant Scheme
Publisher
Bentham Science Publishers Ltd.
Reference32 articles.
1. Harding M.C.; Sloan C.D.; Merrill R.M.; Harding T.M.; Thacker B.J.; Thacker E.L.; MP67: transition from cardiovascular disease to cancer as the leading cause of death in US States, 1999-2013. Circulation 2016,133(Suppl. 1),AMP67-AMP67
2. Andresen T.L.; Jensen S.S.; Jørgensen K.; Advanced strategies in liposomal cancer therapy: problems and prospects of active and tumor specific drug release. Prog Lipid Res 2005,44(1),68-97
3. Bhushan S.; Kakkar V.; Pal H.C.; Mondhe D.M.; Kaur I.P.; The augmented anticancer potential of AP9-cd loaded solid lipid nanoparticles in human leukemia Molt-4 cells and experimental tumor. Chem Biol Interact 2016,244,84-93
4. Guo J.; Wang Y.; Wang J.; Zheng X.; Chang D.; Wang S.; Jiang T.; A novel nanogel delivery of poly-α, β-polyasparthydrazide
by reverse microemulsion and its redox-responsive release of 5-
fluorouridine. Asian J Pharm Sci 2016,735-743
5. Braddock M.; Nanomedicines: design, delivery and detection 2016,48
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献