Affiliation:
1. Department of Hepatobiliary Surgery,
The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
2. Department of
gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
3. Baiyun Hospital of The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
4. The First Clinical Medical School, Guangzhou University of Chinese Medicine, Guangzhou, China
5. Lingnan Medical
Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Abstract
Objective:
The objective of this study is to explore the potential anti-liver cancer mechanism
of Huachansu injection through integrated bioinformatics analysis.
Methods:
Active ingredients of Huachansu injection (extraction of toad skin) were obtained, and
their potential drug targets were predicted via SwissTargetPrediction database. Liver cancer disease
targets were identified from the GEO (Gene Expression Omnibus) dataset and four public databases.
Then Protein-Protein Interaction (PPI) network of toad skin was constructed. GO (Gene Ontology)
enrichment analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis
were performed subsequently. Finally, molecular docking was performed using Auto Dock Vina.
Results:
In the search for therapeutic targets, twenty active components of toad skin were screened
for further study, five hundred and sixty-eight targets of components were identified. In the search for
disease targets, three thousand two hundred and twenty-seven genes were identified after removal of
duplicated genes, one hundred and fifty-nine genes were up-regulated in liver cancer samples while
two hundred and seventy-eight were down-regulated in liver cancer patients. After predicting the
therapeutic targets of the components, the results were cross-checked with the disease targets, thirteen
up-regulated targets and ten down-regulated targets were obtained. Finally, in the results of molecular
docking, seven targets (CDK1, AKR1B1, MMP12, AURKB, CHEK1, AURKA, TTK) were
potential up-regulated targets, three targets (SHBG, SRD5A2, NR1I2) were potential down-regulated
targets, all of which have the best binding energy and molecular interactions.
Conclusion:
CDK1, AKR1B1, MMP12, AURKB, CHEK1, AURKA, and TTK could be potential upregulated
target proteins of Huachansu injection for treating liver cancer. The mechanism of Huachansu
injection in the treatment of liver cancer through these up-regulated targets is related to cell cycle, cellular
senescence, viral carcinogenesis, p53 signaling pathway. SHBG, SRD5A2, and NR1I2 could be potential
down-regulated target proteins of Huachansu injection in treating liver cancer.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Guangdong Province, China
Key Projects of Educational Commission of Guangdong Province, China
Administration of Traditional Chinese Medicine of Guangdong Province, China
Medical innovation project of the First Affiliated Hospital of Guangzhou University of Chinese Medicine
Guangzhou University of Chinese Medicine first-class discipline Construction Research Key Project
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology (medical),Cancer Research,Drug Discovery,Oncology,General Medicine
Cited by
2 articles.
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