Affiliation:
1. Endocrinology Department, TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of
Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China
2. Intensive Care Unit, TCM Regulating
Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine,
Chengdu 610075, China
3. Endocrinology Department, Chongqing Traditional Chinese Medicine Hospital, Chongqing,
400000, China
Abstract
Background:
In China, traditional Chinese medicine (TCM) has been used to treat type 2
diabetes mellitus (T2DM) for centuries.
Methods:
To investigate how the TCM ShenQi (SQC) formulation differs from metformin, four rat
groups, including control, model, T2DM rats treated using SQC (SQC group), and T2DM rats treated
using metformin (Met group), were constructed. The differentially expressed genes (DEGs) between
SQC and metformin groups were screened, and the co-expression modules of the DEGs were constructed
based on the weighted correlation network analysis (WGCNA) method. The correlation between
modules and metabolic pathways was also calculated. The potential gene targets of SQC were obtained
via the TCM systems pharmacology analysis.
Results:
A total of 962 DEGs between SQC and Met groups were screened, and these DEGs were significantly
enriched in various functions, such as sensory perception of the chemical stimulus, NADH
dehydrogenase (ubiquinone) activity, and positive regulation of the fatty acid metabolic process. In
addition, seven co-expression modules were constructed after the redundancy-reduced process. Four of
these modules involved specific activated or inhibited metabolic pathways. Moreover, 334 effective
ingredients of SQC herbs were collected, and four genes (RNASE1 (ribonuclease A family member 1,
pancreatic), ADRB1 (adrenoceptor beta 1), PPIF (peptidylprolyl isomerase F), and ALDH1B1 (aldehyde
dehydrogenase 1 family member B1)) were identified as potential targets of SQC.
Conclusion:
Comparing SQC with metformin to treat T2DM rats revealed several potential gene targets.
These genes provide clues for elucidating the therapeutic mechanisms of SQC.
Funder
National Natural Science Foundation of China
Sichuan Provincial Administration of Traditional Chinese Medicine Special Key Project of Traditional Chinese Medicine Research
Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine
Publisher
Bentham Science Publishers Ltd.
Subject
Cardiology and Cardiovascular Medicine,Pharmacology
Cited by
2 articles.
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