Affiliation:
1. Centre of Excellence in Unani Medicine (Pharmacognosy & Pharmacology), Bioactive Natural Product Laboratory,
School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, India
2. Department of Pharmaceutics,
School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi, India
Abstract
Background:
Bioautography is a technique for the detection of biological activity that
combines the elements of planar chromatography. Its hyphenated variants are widely used in the
screening of natural products possessing biological activity. It can be used in the activity-based
screening of phytochemical ingredients by employing various enzyme processes and reactions and
facilitates the rapid determination of bioactive compounds in pant samples.
Objective:
To give a comprehensive overview of effect-directed assays and biological detection
approaches used in conjugation with thin layer chromatography technique. The present review article
attempts to throw light on the various aspects of bioautography, including its types and applications,
thereby giving its concise overview and its relevance in the field of natural product screening.
Methods:
Various search engines were used for the literature survey, including Google Scholar,
Semantic Scholar, PubMed, ResearchGate and Scopus.
Results:
Bioautography has wide-ranging uses in the screening of compounds such as antioxidants,
antifungals, antimicrobials, estrogenic, antitumors, and various enzyme inhibitors compounds like
α and β-glucosidase inhibitors and α-amylase inhibitors.
Conclusion:
Bioautography serves to be an effective tool for the isolation of bioactive phytochemicals,
thereby allowing us to scientifically validate the biological activities of various compounds,
which can then be utilized for making potent medications for various diseases.
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine