Ultrastructural Analysis of Human Gallstones using Synchrotron Radiation µCT
-
Published:2019-05-03
Issue:1
Volume:22
Page:13-17
-
ISSN:1386-2073
-
Container-title:Combinatorial Chemistry & High Throughput Screening
-
language:en
-
Short-container-title:CCHTS
Author:
Chen Weixin1, Liu Riming2, Tao Suo3, Shen Weixing4, Zhou Weihong4, Song Chao3, Lu Huanhua4, Xing Chungen1
Affiliation:
1. Department of General Surgery, The Second Affiliated Hospital of Soochow University, No.1055, Sanxiang Rd, Suzhou 215004, China 2. Department of Central Laboratory, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, Shandong Province, China 3. The General Surgery Department of Zhongshan Hospital, The General Surgery Institute, Fudan University, 180 Fenglin Road, Xuhui, China 4. Department of General Surgery, Affiliated Zhongshan Hospital of Fudan University, Qinpu Branch, Qingpu, Shanghai, 201700, China
Abstract
Objective:
Gallstone formation is a pathological process of mineralization in the human
body. Determination of the morphology and ultrastructure of gallstones holds the key to
understanding the pathophysiology of gallbladder disease. Synchrotron radiation phase-contrast Xray
microtomography is a novel technology, which is designed for comprehensive analysis of
gallstone ultrastructure.
Materials and Methods:
Nine human gallstones were obtained from the Department of Pathology,
Qingpu branch of Zhongshan Hospital Affiliated to Fudan University (China), and scanned by
synchrotron radiation µCT (SR µCT). The imaging data generated by SR µCT scan were analyzed.
Results:
The three-dimensional ultrastructure of human gallstones corresponding to their
cholesterol and bile pigment composition was determined.
Conclusions:
The ultrastructure of gallstones exhibits considerable diversity and complexity. The
synchrotron radiation phase-contrast X-ray microtomography is a valuable tool for in-depth study
of human gallstones.
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine
Reference17 articles.
1. Van Erpecum KJ. Clin Res Hepatol Gastroenterol, Pathogenesis of cholesterol and pigment gallstones: An update.,, 2011, 35,, 281-287, 2. Abeysuriya V, Deen KI, Navarathne NM. Hepatobiliary Pancreat Dis Int, Biliary microlithiasis, sludge, crystals, microcrystallization, and usefulness of assessment of nucleation time.,, 2010, 9,, 248-253, 3. Halldestam I, Kullman E, Borch K. Br J Surg, Incidence of and potential risk factors for gallstone disease in a general population sample.,, 2009, 96,, 1315-1322, 4. Ravnborg L, Teilum D, Pedersen LR. Scand J Gastroenterol, Gallbladder stones classified by chemical analysis of cholesterol content. Frederiksberg, 1987-1988.,, 1990, 25,, 720-724, 5. Sutton MD, Garwood RJ, Siveter DJ. Palaeontol Electronica, SPIERS and VAXML a software toolkit for tomographic visualisation and a format for virtual specimen interchange.,, 2012, 2,, 396-396,
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|