Salmonella Isolated From Raw Chicken Meats at Selected Slaughterhouses in Peninsular Malaysia; Their Antibiotic Resistance Profiles and Biofilm Formation on Nutrient-Limited Media

Author:

Ismail Zuraidah,Azmi Nur Naqiyah,Mahyudin Nor Ainy,Wan Omar Wan Hasyera,Abdul Rahman Marina,Sapar Marni

Abstract

Salmonella is one of the pathogens responsible for foodborne diseases. Antibiotic resistance of Salmonella, particularly multidrug-resistant (MDR) strains have emerged and are becoming more prevalent, which is a very serious issue worldwide. This study sought to determine the antibiotic resistance profiles of Salmonella isolated from raw chicken meats, which were collected at selected slaughterhouses in Peninsular Malaysia and evaluating its biofilm-forming capability on surfaces. Antibiotic resistance of 135 Salmonella isolates against 12 antibiotics were investigated via disk diffusion method. The biofilm-forming ability of the isolates was evaluated by crystal violet staining using two media; a tryptic soy broth (TSB) and a 1/20 TSB with incubation periods of 24 and 48 h at 37 °C. A total of 118 strains of Salmonella showed higher resistance to erythromycin (87.41%), followed by tetracycline (85.19%;); 93 of the isolates (68.88%) were multi-drug resistant. A greater quantity of Salmonella was able to produce biofilm when grown in 1/20-TSB (90.37%) compared to the growth in TSB (88.15%), respectively. The findings in this study showed high prevalence, antibiotic resistance, and the biofilm forming ability of Salmonella strains isolated from raw chicken meats, suggesting that effective measures are required to ensure food safety in the poultry industry.

Publisher

Persatuan Biologi Gunaan Malaysia

Reference63 articles.

1. Adzitey, F., Rusul, G. & Huda, N. 2012. Prevalence and antibiotic resistance of Salmonella serovars in ducks, duck rearing and processing environments in Penang, Malaysia. Food Research International, 45(2): 947-952.

2. Agarwal, R.K., Singh, S., Bhilegaonkar, K.N. & Singh, V.P. 2011. Optimization of microtitre plate assay for the testing of biofilm formation ability in different Salmonella serotypes. International Food Research Journal, 18(4): 1493-1498.

3. Ariffin, A.S., Mohtar, S. & Baluch, N.H. 2014. Broiler industry with emphasis on short supply chain in Malaysia. Paper presented at the meeting of the 4th International Conference on Technology and Operation Management (ICTOM 04), Kuala Lumpur. August 2014.

4. Bacci, C., Boni, E., Alpigiani, I., Lanzoni, E., Bonardi, S. & Brindani, F. 2012. Phenotypic and genotypic features of antibiotic resistance in Salmonella enterica isolated from chicken meat and chicken and quail carcasses. International Journal of Food Microbiology, 160(1): 16-23.

5. Beyene, T., Yibeltie, H., Chebo, B., Abunna, F., Beyi, A.F., Mammo, B., Ayana, D. & Duguma, R. 2016. Identification and antimicrobial susceptibility profile of Salmonella isolated from selected dairy farms, abattoir and humans at Asella Town. Journal of Veterinary Science and Technology, 7(3): 1-7.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3