The expression levels of MIR375 and MIR451a genes in subacute thyroiditis

Abstract

Purpose: The most typical reason for thyroid pain is subacute thyroiditis (SAT). Incidence of SAT is higher in women than in men and the average annual frequency of SAT is 12.1 cases per 100,000 people. Endogenous, brief, single-stranded non-coding RNAs, called micro RNAs (miRNAs), are inhibitors of gene expression. In the posttranscriptional regulation of gene expression, miRNAs are essential. In this investigation, we explored the potential utility of two circulating miRNAs, MIR451A and MIR375, as markers for the detection and monitoring of SAT. Materials and Methods: Fifty SAT patients and 41 healthy people were enrolled in this study. Using the quantitative real-time polymerase chain reaction (qRT-PCR) approach, miRNA expression levels were assessed. The 2-ΔΔCt technique was used to calculate miRNA expression levels. The t-test was used to assess the statistical significance of miRNA expression. Results: While MIR451A expression levels were discovered to vary between healthy controls and SAT patients, MIR375-3p expression levels were discovered to be similar in both groups. MIR375-3p and MIR451A expression levels were shown to be similarly in different SAT stages (hyperthyroid, euthyroid, and hypothyroid). The expression levels of MIR375-3p and MIR451A were analyzed for association with the clinical characteristics of SAT patients, but no correlation was observed. Conclusion: In this study, it was found that the expression level of circulating MIR451A was lower in SAT patients, which shows that MIR451A might be effective in the SAT disease's onset.