Exploring the antibacterial potential of tunicate-associated bacteria (Ascidiacea) at the shipwreck site of Menjangan Kecil Waters, Karimunjawa

Author:

Hendrawati Aen1,Ayuningrum Diah2,Sabdaningsih Aninditia2,Amalia Rosa3

Affiliation:

1. Student of Department of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50241, Indonesia

2. Department of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50241, Indonesia

3. Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang 50241, Indonesia

Abstract

Menjangan Kecil Island is situated south of Karimunjawa Island and boasts a rich diversity of coral reefs and associated marine life, including tunicates. Tunicates, also known as sea squirts, produce secondary metabolites to defend against predators. This study aims to explore the antibacterial potential of tunicate-associated bacteria from shipwrecks in Menjangan Kecil Waters. The research adopts an exploratory descriptive approach. Seventy-two bacterial isolates were recovered from five tunicate specimens. Fifteen isolates exhibited antibacterial activity against Vibrio harveyi, with two isolates, KJ1.3.02.05 and KJ1.4.02.02, displaying the highest activity. Molecular identification via 16S rRNA gene amplification revealed that KJ1.3.02.05 was Sinomicrobium oceani (99.64% similarity), and KJ1.4.02.02 was Bacillus haynesii (98.64% similarity).

Funder

Universitas Diponegoro

Publisher

Malaysian Society for Molecular Biology and Biotechnology

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