Affiliation:
1. Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangzhou China
2. Germany Reference Laboratory for KHVD, Institute of Infectology, Friedrich‐Loffler‐Institut (FLI) Federal Research Institute for Animal Health Greifswald–Insel Riems Germany
Abstract
AbstractIn this issue, we established rapid, cost‐effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA‐LFD) and real‐time RPA for cyprinid herpesvirus 3(CyHV‐3), and evaluated their sensitivity, specificity, and applicability, the real‐time RPA method could achieve sensitive diagnosis of CyHV‐3 within 1.3 copies per reaction, respectively. The real‐time RPA method is 10‐fold more sensitive than RPA‐LFD method. The exact number of CyHV‐3 can be calculated in each sample by real‐time RPA. The sera from koi also can be tested in these methods. In addition, no cross‐reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV‐1), cyprinid herpesvirus 2(CyHV‐2), type I grass carp reovirus (GCRV‐I), type II GCRV (GCRV‐II), type III GCRV (GCRV‐III), and Aeromonas hydrophila.
Funder
Earmarked Fund for China Agriculture Research System