RvD1 disrupts nociceptor neuron and macrophage activation and neuroimmune communication, reducing pain and inflammation in gouty arthritis in mice

Abstract

Background and PurposeGouty arthritis is characterized by an intense inflammatory response to monosodium urate crystals (MSU), which induces severe pain. Current therapies are often ineffective in reducing gout‐related pain. Resolvin D1 (RvD1) is a specialized pro‐resolving lipid mediator with anti‐inflammatory and analgesic proprieties. In this study, we evaluated the effects and mechanisms of action of RvD1 in an experimental mouse model of gouty arthritis, an aim that was not pursued previously in the literature.Experimental ApproachMale mice were treated with RvD1 (intrathecally or intraperitoneally) before or after intraarticular stimulation with MSU. Mechanical hyperalgesia was assessed using an electronic von Frey aesthesiometer. Leukocyte recruitment was determined by knee joint wash cell counting and immunofluorescence. IL‐1β production was measured by ELISA. Phosphorylated NF‐kB and apoptosis‐associated speck‐like protein containing CARD (ASC) were detected by immunofluorescence, and mRNA expression was determined by RT‐qPCR. CGRP release was determined by EIA and immunofluorescence. MSU crystal phagocytosis was evaluated by confocal microscopy.Key ResultsRvD1 inhibited MSU‐induced mechanical hyperalgesia in a dose‐ and time‐dependent manner by reducing leukocyte recruitment and IL‐1β production in the knee joint. Intrathecal RvD1 reduced the activation of peptidergic neurons and macrophages as well as silenced nociceptor to macrophage communication and macrophage function. CGRP stimulated MSU phagocytosis and IL‐1β production by macrophages. RvD1 downmodulated this phenomenon directly by acting on macrophages, and indirectly by inhibiting CGRP release and CGRP‐dependent activation of macrophages.Conclusions and ImplicationsThis study reveals a hitherto unknown neuro‐immune axis in gouty arthritis that is targeted by RvD1.