X‐Ray and Neutron Small‐Angle Scattering Studies of the Complex between Protein S1 and the 30‐S Ribosomal Subunit

Abstract

X‐ray and neutron solution scattering experiments have been done to investigate the influence of the binding of ribosomal protein S1 on the conformation of the 30‐S ribosomal subunit of Escherichia coli. The following conclusions were made. The alterations (if any) in conformation of the non‐S1 parts of the 30‐S subunit induced by S1 binding are too small to be detected (< 0.1 nm change in radius of gyration). The center of gravity of protein S1 bound to the 30‐S subunit is quite far from the center of gravity of the particle (≅ 7.5 nm).