Affiliation:
1. Department of Dentistry Federal University of Bahia (UFBA) Salvador Bahia Brazil
2. Department of Dentistry Bahiana School of Medicine and Public Health Salvador Bahia Brazil
3. Department of Biochemistry and Biophysics Institute of Health Science, Federal University of Bahia (UFBA) Salvador Bahia Brazil
4. Department of Biointeraction Federal University of Bahia (UFBA) Salvador Bahia Brazil
5. Department of Health State University of Feira de Santana (UEFS) Feira de Santana Bahia Brazil
Abstract
AbstractObjectiveThis observational study aimed to explore the metagenomics of subgingival biofilms in individuals with varying degrees of asthma, from severe to none, to elucidate the association between the subgingival microbiome and asthma.Materials and MethodsSubgingival biofilm samples were collected from thirty participants at the Asthma Control Program Outpatient Clinic in Bahia (ProAR). These samples were categorized into six groups based on the severity of asthma and the presence or absence of periodontitis. We employed next‐generation sequencing (Illumina MiSeq), targeting the 16S rRNA gene, to characterize the microbial communities present. Our analysis included descriptive statistics and sequencing data, evaluated using multivariate statistical methods such as the Shannon index, principal coordinate analysis, and the Bray–Curtis dissimilarity.ResultsOur findings indicate a higher prevalence of periodontally detrimental bacterial genera in individuals with severe asthma and periodontitis. Additionally, individuals with asthma, but without periodontitis, exhibited a tendency toward dysbiosis, particularly in cases of severe asthma.ConclusionThis research provides new insights into the composition of the subgingival microbiome in individuals with varying severities of asthma and periodontitis. The genera identified in this study underscore the need for further investigations to build upon these findings.