Embedding of exogenous B cell epitopes on the surface of UreB structure generates a broadly reactive antibody response against Helicobacter pylori

Author:

Ji Qianyu1,Ma Junfei2,Wang Shuying1,Liu Qing13ORCID

Affiliation:

1. School of Health Science and Engineering University of Shanghai for Science and Technology Shanghai China

2. College of Agriculture and Forestry Linyi University Linyi China

3. Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Qingdao China

Abstract

AbstractSince Helicobacter pylori (H. pylori) resistance to antibiotic regimens has increased, vaccination is becoming an increasingly important alternative therapy to control H. pylori infection. UreB, FlaA, AlpB, SabA, and HpaA proteins of H. pylori were previously proved to be used as candidate vaccine antigens. Here, we developed an engineered antigen based on a recombinant chimeric protein containing a structural scaffold from UreB and B cell epitopes from FlaA, AlpB, SabA, and HpaA. The multi‐epitope chimeric antigen, named MECU, could generate a broadly reactive antibody response including antigen‐specific antibodies and neutralising antibodies against H. pylori urease and adhesins. Moreover, therapeutic immunisation with MECU could reduce H. pylori colonisation in the stomach and protect the stomach in BALB/c mice. This study not only provides promising immunotherapy to control H. pylori infection but also offers a reference for antigen engineering against other pathogens.

Publisher

Wiley

Subject

Immunology,Immunology and Allergy

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