Analytical validation of a flow cytometric protocol for quantification of platelet microparticles in dogs

Author:

Cremer Signe E.1ORCID,Krogh Anne K. H.1,Hedström Matilda E. K.1,Christiansen Liselotte B.1,Tarnow Inge2,Kristensen Annemarie T.1

Affiliation:

1. Department of Veterinary and Animal Sciences; University of Copenhagen; Copenhagen Denmark

2. Savara Pharmaceuticals; Hørsholm Denmark

Funder

Danish fund ‘Fondet for Sygdomsbekaempelse hos Vore Familiedyr’

Publisher

Wiley

Subject

General Veterinary

Reference40 articles.

1. Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation;Berckmans;Thromb Haemost,2001

2. Assessment of in vitro-generated platelet microparticles using a modified flow cytometric strategy;Tocchetti;Thromb Res,2001

3. Association of factor V activity with membranous vesicles released from human platelets: requirement for platelet stimulation;Bode;Thromb Res,1985

4. Complement proteins C5b-9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity;Sims;J Biol Chem,1988

5. Flow cytometric measurement of microparticles: pitfalls and protocol modifications;Shah;Platelets,2008

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