ZmRPN1 confers quantitative variation in pollen number and boosts hybrid seed production in maize

Author:

Guo Ting1,Jiang Lu‐Guang1,Li Bo1,Jiang Huan1,Zheng Tong‐Xin1,Luo Jin‐Hong1,He Yan1ORCID

Affiliation:

1. MOE Key Laboratory of Crop Heterosis and Utilization, National Maize Improvement Center of China China Agricultural University Beijing China

Abstract

SummaryThe number of pollen grains is a critical determinant of reproductive success in seed plants and varies among species and individuals. However, in contrast with many mutant‐screening studies relevant to anther and pollen development, the natural genetic basis for variations in pollen number remains largely unexplored. To address this issue, we carried out a genome‐wide association study in maize, ultimately revealing that a large presence/absence variation in the promoter region of ZmRPN1 alters its expression level and thereby contributes to pollen number variation. Molecular analyses showed that ZmRPN1 interacts with ZmMSP1, which is known as a germline cell number regulator, and facilitates ZmMSP1 localization to the plasma membrane. Importantly, ZmRPN1 dysfunction resulted in a substantial increase in pollen number, consequently boosting seed production by increasing female–male planting ratio. Together, our findings uncover a key gene controlling pollen number, and therefore, modulation of ZmRPN1 expression could be efficiently used to develop elite pollinators for modern hybrid maize breeding.

Funder

National Basic Research Program of China

Publisher

Wiley

Subject

Plant Science,Agronomy and Crop Science,Biotechnology

Reference54 articles.

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