IRS1 promotes thyroid cancer metastasis through EMT and PI3K/AKT pathways

Abstract

AbstractObjectiveInsulin receptor substract 1 (IRS1) protein is an important signal transduction adapter for extracellular signal transduction from insulin‐like growth factor‐1 receptor and its family members to IRS1 downstream proteins. IRS1 has been reported to be involved in tumourigenesis and metastasis in some of solid tumors. Investigating the role of IRS1 in thyroid cancer can help to screen high risk patients at the initial diagnosis.Design, Patients and MeasurementsImmunohistochemical assay was used to detect the expression levels of IRS1 in 131 metastatic thyroid cancer tissues. Wound healing, cell invasion and colony formation assays were used to study the functions of IRS1 in vitro. RNA sequencing (RNA‐seq) and Western blot analysis analyses were performed to examine the underlying regulation mechanisms of IRS1 in thyroid cancer cells.ResultsIRS1 was highly expressed in thyroid cancers and its expression was positively associated with distant metastasis and advanced clinical stages. In vitro studies demonstrated that IRS1 is an important mediator of migration, invasion and colony formation of thyroid cancer cells. RNA‐seq showed that IRS1 promoted the metastasis of thyroid cancer by regulating epithelial‐mesenchymal transition and phosphoinositide 3‐kinase (PI3K)/AKT pathway.ConclusionsIRS1 overexpression contributes to the aggressiveness of thyroid cancer and is expected to be a stratified marker and a potential therapeutic target for thyroid cancer.